Next-gen Sequencing Certification Course

The Syllabus Covers

Introduction to NGS
Principles of NGS: Illumina, PacBio, Oxford Nanopore.
Applications: whole-genome sequencing, RNA-Seq, metagenomics.
Reading: NGS technology reviews.

Sample Preparation and Sequencing
Library preparation, quality control, sequencing workflows.
Practical: Simulate library preparation steps.

NGS Data Processing
Quality control: FastQC, trimming.
Alignment: BWA, Bowtie.
Practical: Process raw NGS data using FastQC.

Data Analysis and Variant Calling
Tools: GATK, SAMtools, VarScan.
Variant annotation and interpretation.
Practical: Perform variant calling on a genomic dataset.

Advanced Applications and Interpretation
RNA-Seq, epigenomics, single-cell sequencing.
Data visualization and biological interpretation.
Practical: Analyze a single-cell RNA-Seq dataset.