Cellculture Technique Course

The Course Modules covers

⦁ Introduction to Cell Culture
⦁ Basics of cell culture: primary vs. immortalized cells, adherent vs. suspension cultures.
⦁ Sterile techniques and biosafety.
⦁ Equipment: biosafety cabinets, incubators, microscopes.
⦁ Practical: Set up a sterile workspace.

⦁ Cell Culture Media and Growth Conditions
⦁ Types of media (DMEM, RPMI, etc.), supplements (FBS, antibiotics).
⦁ Optimizing pH, CO2, and temperature.
⦁ Practical: Prepare and filter-sterilize cell culture media.

⦁ Cell Maintenance and Subculturing
⦁ Passaging cells, cell counting (hemocytometer, automated counters).
⦁ Cryopreservation and thawing techniques.
⦁ Practical: Subculture a cell line (e.g., HeLa) and perform cell counting.

⦁ Applications and Assays in Cell Culture
⦁ Common assays: MTT, viability, apoptosis, and proliferation assays.
⦁ Applications in drug screening, toxicology, and cancer research.
⦁ Practical: Perform an MTT assay to assess cell viability.

⦁ Troubleshooting and Contamination Control
⦁ Identifying and preventing contamination (bacterial, fungal, mycoplasma).
⦁ Troubleshooting poor cell growth or unexpected results.
⦁ Practical: Test for mycoplasma contamination using PCR.