Next-gen Sequencing Certification Course – Self Learning

The Learning Units

⦁ Introduction to NGS
⦁ Principles of NGS: Illumina, PacBio, Oxford Nanopore.
⦁ Applications: whole-genome sequencing, RNA-Seq, metagenomics.
⦁ Reading: NGS technology reviews.

⦁ Sample Preparation and Sequencing
⦁ Library preparation, quality control, sequencing workflows.
⦁ Practical: Simulate library preparation steps.

⦁ NGS Data Processing
⦁ Quality control: FastQC, trimming.
⦁ Alignment: BWA, Bowtie.
⦁ Practical: Process raw NGS data using FastQC.

⦁ Data Analysis and Variant Calling
⦁ Tools: GATK, SAMtools, VarScan.
⦁ Variant annotation and interpretation.
⦁ Practical: Perform variant calling on a genomic dataset.

⦁ Advanced Applications and Interpretation
⦁ RNA-Seq, epigenomics, single-cell sequencing.
⦁ Data visualization and biological interpretation.
⦁ Practical: Analyze a single-cell RNA-Seq dataset.