Next-gen Sequencing Certification Course
The Syllabus Covers Introduction to NGSPrinciples of NGS: Illumina, PacBio, Oxford Nanopore.Applications: whole-genome sequencing, RNA-Seq, metagenomics.Reading: NGS…
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The Syllabus Covers
Introduction to NGS
Principles of NGS: Illumina, PacBio, Oxford Nanopore.
Applications: whole-genome sequencing, RNA-Seq, metagenomics.
Reading: NGS technology reviews.
Sample Preparation and Sequencing
Library preparation, quality control, sequencing workflows.
Practical: Simulate library preparation steps.
NGS Data Processing
Quality control: FastQC, trimming.
Alignment: BWA, Bowtie.
Practical: Process raw NGS data using FastQC.
Data Analysis and Variant Calling
Tools: GATK, SAMtools, VarScan.
Variant annotation and interpretation.
Practical: Perform variant calling on a genomic dataset.
Advanced Applications and Interpretation
RNA-Seq, epigenomics, single-cell sequencing.
Data visualization and biological interpretation.
Practical: Analyze a single-cell RNA-Seq dataset.
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