Certification Course in Molecular Biology Techniques

The Training Modules contains

⦁ Molecular Biology Techniques
⦁ Introduction to Molecular Biology
⦁ Central dogma, DNA/RNA/protein basics.
⦁ Overview of techniques: PCR, cloning, sequencing.
⦁ Lab safety and molecular biology workflows.

⦁ DNA Extraction and Quantification)
⦁ Genomic and plasmid DNA isolation methods.
⦁ Quantification: Nanodrop, Qubit, gel electrophoresis.
⦁ Practical: Extract DNA from a biological sample.
⦁ Polymerase Chain Reaction (PCR)
⦁ PCR principles, primer design, and optimization.
⦁ Variants: RT-PCR, qPCR, multiplex PCR.
⦁ Practical: Amplify a target gene using PCR.

⦁ Cloning and Gene Expression
⦁ Restriction digestion, ligation, and transformation.
⦁ Expression systems: bacterial, mammalian, yeast.
⦁ Practical: Clone a gene into a plasmid vector.

⦁ Advanced Techniques and Applications
⦁ CRISPR-Cas9, RNA interference, and protein expression analysis.
⦁ Applications: gene editing, functional genomics.
⦁ Practical: Design a CRISPR experiment for gene knockout.